ACTB (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human ACTB full-length ORF ( AAH01301, 1 a.a. - 375 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MDDDIAALVVDNGSGMCKAGFAGDDAPRAVSPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERRYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
66.99
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — ACTB
Entrez GeneID
60GeneBank Accession#
BC001301Protein Accession#
AAH01301Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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Interactome
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Pathway
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Disease
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Publication Reference
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Yin and Yang Regulation of Liver X Receptor α Signaling Control of Cholesterol Metabolism by Poly(ADP-ribose) polymerase 1.
Fengxiao Zhang, Cheng Wang, Yuhan Jiang, Kun Huang, Fangmei Liu, Meng Du, Xi Luo, Dan Huang, Kai Huang.
International Journal of Biological Sciences 2020 Sep; 16(15):2868.
Application:PI, N/A, Recombinant protein.
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Poly(ADP-ribose) Polymerase 1 Is Indispensable for Transforming Growth Factor-beta Induced Smad3 Activation in Vascular Smooth Muscle Cell.
Huang D, Wang Y, Wang L, Zhang F, Deng S, Wang R, Zhang Y, Huang K.
PLoS One 2011 Oct; 6(10):e27123.
Application:Far-Western blot, Rat, Vascular smooth muscle cells.
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Yin and Yang Regulation of Liver X Receptor α Signaling Control of Cholesterol Metabolism by Poly(ADP-ribose) polymerase 1.
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