KRAS/CEN12q FISH Probe
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Specification
Product Description
Labeled FISH probes for identification of gene amplification using Fluorescent In Situ Hybridization Technique. (Technology).
Probe 1
Name: KRAS
Size: Approximately 250kb
Fluorophore: Texas Red
Location: 12p12Probe 2
Name: CEN12q
Size: Approximately 580kb
Fluorophore: FITC
Location: 12q12Probe Gap
The gap between two probes is approximately 17,900 kb.
Origin
Human
Source
Genomic DNA
Reactivity
Human
Form
Liquid
Notice
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Regulation Status
For research use only (RUO)
Quality Control Testing
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Supplied Product
DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe
Storage Instruction
Store at 4°C in the dark.
Note
Hybridization position of the probes on the chromosome. -
Applications
Fluorescent In Situ Hybridization (Cell)
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Human uterus cancer (FFPE) stained with KRAS/CEN12q FISH Probe. Human uterus cancer showed KRAS gene amplification.Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Human colon cancer (FFPE) stained with KRAS/CEN12q FISH Probe. Human colon cancer showed no KRAS gene amplification. -
Gene Info — KRAS
Entrez GeneID
3845Gene Name
KRAS
Gene Alias
C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2
Gene Description
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene Ontology
HyperlinkGene Summary
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq
Other Designations
K-ras p21 protein|Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog|PR310 c-K-ras oncogene|c-K-ras2 protein|c-Kirsten-ras protein|cellular c-Ki-ras2 proto-oncogene|oncogene KRAS2|transforming protein p21|v-Ki-ras2 Kirsten rat sarcoma 2 viral oncoge
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Interactome
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Pathway
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Disease
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Publication Reference
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KIF13B-NRG1 Gene Fusion and KRAS Amplification in a Case of Natural Progression of Lung Cancer.
Xia D, Le LP, Iafrate AJ, Lennerz J.
Amino Acids 2017 Apr; 25(3):238.
Application:FISH, Human , Human lung cancer.
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Clonal evolution and KRAS-MET coamplification during secondary resistance to EGFR-targeted therapy in metastatic colorectal cancer.
Bianchi AS, Valtorta E, Amatua A , Veronese S, Lauricella C, Bonazzina E, Siravegna G, Truini M, Bardelli A, Siena S.
ESMO Open 2016 Aug; 1(4):e000079.
Application:FISH, Human, Colorectal cancer.
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KIF13B-NRG1 Gene Fusion and KRAS Amplification in a Case of Natural Progression of Lung Cancer.
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