Small RNAs, miRNA and siRNA, have drawn much attention recently. The Small RNA Marker Easy has five single-stranded RNAs, 20, 30, 40, 50 and 100 bases, which is useful for a research of small RNAs. The 20, 30, 40 and 50 bases are synthesized by chemically (non phosphorylated). The 100 bases is synthesized by in vitro transcription. The Small RNA Marker Easy is supplied in a ready-to-use mixture of loading dye and RNAs (containing formamide, EDTA sodium salt, bromphenol blue). It is manufactured for denaturing polyacrylamide gel electrophoresis. The Small RNA Marker Easy can be visualized by UV light exposure after ethidium bromide staining.
Quality Control Testing:
After 18 hrs incubation of the Small RNA Marker Easy at 37°C, no visible degradation of the marker is observed in 12.5 % polyacrylamide / 7.5 M urea gel electrophoresis.
RNA Loading buffer PA
Store at -80 °C. Repeated freeze/thaw cycles should be avoided.
The Small RNA Marker Easy is not prepared for estimating of RNA amount. RNA is very sensitive to degradation by nucleases. To avoid damaging the Small RNA Marker Easy, use extreme care during manipulations to prevent nuclease contamination. Wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyrocarbonate (DEPC). Nuclease-free disposable plasticware should be used. Solutions and reagents to mix the marker should be high grade and nuclease-free. To use, thaw the Small RNA Marker Easy on ice and keep it on ice while using. For heat denaturation, transfer aliquot of the Small RNA Marker Easy to another tube, then heat it. Avoid repeated heat denaturizing. Formamide is suspected to be harmful. It is irritate to the eyes and skin. Wear appropriate gloves and safety glasses. Put a lid tightly at the time of storage.