The RNA Marker Low consists of seven single-stranded RNAs. The 20-base and 50-base RNA are synthesized by chemically (not phosphorylated). The 100, 200, 300, 400 and 500 bases are synthesized by in vitro transcription. The RNA Marker Low is suitable for determinating size of single-stranded RNAs in denaturing polyacrylamide gel electrophoresis. The concentration of each RNA (20-500 base) in the marker is approximately 0.1 ug/ul. It is useful for estimating of RNA amount. The RNA Marker Low can be visualized by ethidium bromide staining.
Quality Control Testing:
After 18 hr incubation of the RNA Marker Low at 37°C, no visible degradation of the marker is observed in 5 % polyacrylamide / 8M urea gel electrophoresis.
1 uL (0.7 ug) RNA marker low mix with 5 uL of gel loading buffer.
10 mM Tris-HCl (pH 8.0) buffer containing 1 mM EDTA
Store at -80 °C. Repeated freeze/thaw cycles should be avoided.
RNA is very sensitive to degradation by nucleases. To avoid damaging the RNA Marker Low, use extreme care during manipulations to prevent nuclease contamination. Wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyrocarbonate (DEPC). Nuclease-free disposable plasticware should be used. Solutions and reagents to mix the marker should be high grade and nuclease-free. To use, thaw the RNA Marker Low on ice and keep it on ice while using.