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Last updated: 2014/9/29
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ACTB polyclonal antibody

  • Catalog # : PAB9980
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ACTB.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 359-368 of human ACTB.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:40000)
    Western Blot (1:1000-1:4000)
    Immunofluorescence (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using ACTB polyclonal antibody (Cat # PAB9980) shows detection of a predominant band at ~42 KDa corresponding to ACTB (arrowhead) in various lysates.
    Lysates are as follows : HEK 293 (Lane 2), human lung carcinoma A-549 (Lane 3) and mouse brain (Lane 4) all using the 800 nm channel (green).
    Approximately 35 ug of each lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1 : 1,500.
    Incubation was overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
    Molecular weight markers are shown in lane 1 using the 700 nm channel (red).
    IRDye™800 fluorescence image was captured using the Odyssey u Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of ACTB polyclonal antibody (Cat # PAB9980) at 1 : 200 is shown to detect actin at the neuromuscular junction of rana pipiens tissue (sections 4.2 um thick).
    Personal Communication. S. Moeckel-Cole, Univ. MA, Amherst, MA.
  • ELISA
  • Application Image
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 60
  • Protein Accession#:
  • P60709;NP_001092
  • Gene Name:
  • ACTB
  • Gene Alias:
  • PS1TP5BP1
  • Gene Description:
  • actin, beta
  • Gene Summary:
  • This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
  • Other Designations:
  • PS1TP5-binding protein 1,actin, cytoplasmic 1,beta actin,beta cytoskeletal actin
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