Western blot using JAG1 polyclonal antibody (Cat # PAB9954) shows detection of JAG1 protein in various whole cell lysates : human brain (Lane 1), human kidney (Lane 2), human liver (Lane 3), and mouse liver (Lane 5). Lane 4 contained sample buffer only. The band at ~134 KDa in lane 5 is believed to be JAG1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1 : 500 dilution. The membrane was washed and reacted with a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 KDa.
Immunohistochemical staining of human cervical cancer tissue (40X magnification) using JAG1 polyclonal antibody (Cat # PAB9954) . Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1 : 100 dilution of primary antibody was used. Personal Communication. Martin Kast Laboratory.
Immunofluorescence Microscopy using JAG1 polyclonal antibody (Cat # PAB9954) of human corneal epithelial cells. Primary antibody was used at a 1 : 500 dilution. The JAG1 (green staining) is local-ized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue) . Personal Communication. Aihua Ma, Univdersity of Cardiff.
The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq