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Last updated: 2014/9/29
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JAG1 polyclonal antibody

  • Catalog # : PAB9954
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of JAG1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 110-125 of human JAG1.
  • Host:
  • Rabbit
  • Reactivity:
  • Clawed frog, Dog, Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:100-1:500)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using JAG1 polyclonal antibody (Cat # PAB9954) shows detection of JAG1 protein in various whole cell lysates : human brain (Lane 1), human kidney (Lane 2), human liver (Lane 3), and mouse liver (Lane 5).
    Lane 4 contained sample buffer only.
    The band at ~134 KDa in lane 5 is believed to be JAG1 precursor.
    The identity of minor reactive bands is unknown.
    Each lane contains approximately 20 ug of lysate.
    Primary antibody was used at a 1 : 500 dilution.
    The membrane was washed and reacted with a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG.
    Exposure time was 1 min.
    Predicted molecular weight is 134 KDa.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human cervical cancer tissue (40X magnification) using JAG1 polyclonal antibody (Cat # PAB9954) . Tissue was fixed with formalin and embedded in paraffin.
    Hematoxylin was used to counter-stain cells. A 1 : 100 dilution of primary antibody was used.
    Personal Communication. Martin Kast Laboratory.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence Microscopy using JAG1 polyclonal antibody (Cat # PAB9954) of human corneal epithelial cells.
    Primary antibody was used at a 1 : 500 dilution.
    The JAG1 (green staining) is local-ized to the cytoplasm and is consistent with reports in the literature.
    The nucleus is stained with Bis benzimide (blue) .
    Personal Communication. Aihua Ma, Univdersity of Cardiff.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 182
  • Gene Name:
  • JAG1
  • Gene Alias:
  • AGS,AHD,AWS,CD339,HJ1,JAGL1,MGC104644
  • Gene Description:
  • jagged 1 (Alagille syndrome)
  • Gene Summary:
  • The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000030278,jagged 1
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