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- Home > Products > Polyclonal Ab > Human > Others > AKT1 polyclonal antibody
AKT1 polyclonal antibody
- Catalog # : PAB9931
- Visit Frequency :
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- 200 uL
- USD $ 415
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Email : sales@abnova.com
Phone : +1-909-992-0619 (new)
Fax : +1-909-992-3401- Products will be shipped directly to the customer.
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of AKT1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to amino acids 460-480 of AKT1.
- Sequence:
- CRPHFPQFSYSASGTA
- Host:
- Rabbit
- Reactivity:
- Chicken, Human, Mouse, Rat
- Form:
- Liquid
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:2000-1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:500-1:2000)
Immunofluorescence (1:100-1:1000)
The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.1% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?
Lawlor MA, Alessi DR.J Cell Sci. 2001 Aug;114(Pt 16):2903-10.
- 2.
- Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture.
Alessi DR.Biochem Soc Trans. 2001 May;29(Pt 2):1-14.
- Applications
- Western Blot (Cell lysate)
- Immunoblotting of AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 500 dilution to detect AKT1 by Western blot.
Approximately 20 ug of an NIH/3T3 whole cell lysate was loaded on a 10% NuPage SDS-PAGE gel using MOPS buffer.
After washing, a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Pierce Chemical's Super Signal™ substrate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 1,000 dilution to detect AKT1 by immunohistochemistry. In panel (A) normal colon tissue is stained. Panel (B) shows tumor tissue. Tissue was formalin-fixed and paraffin embedded.
- Immunofluorescence
- Immunofluorescence microscopy of AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT1 construct. AKT1 polyclonal antibody (Cat # PAB9931) staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
