Grin2b (phospho Y125) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k Grin2b subunit of the NMDAR phosphorylated at Tyr1252 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Grin2b (phospho Y125) polyclonal antibody (Cat # PAB9671). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Immunofluorescence
Grin2b (phospho Y125) polyclonal antibody (Cat # PAB9671) and PSD-95 polyclonal antibody (Cat # PAB9623) double-label Immunostaining on 14 DIV Cortical neurons.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Grin2b.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y125 of rat Grin2b.
Host
Rabbit
Theoretical MW (kDa)
180
Reactivity
Bovine, Chicken, Dog, Human, Mouse, Primates, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k Grin2b subunit of the NMDAR phosphorylated at Tyr1252 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Grin2b (phospho Y125) polyclonal antibody (Cat # PAB9671). The immunolabeling is completely eliminated by treatment with lambda-Ptase.Immunofluorescence
Grin2b (phospho Y125) polyclonal antibody (Cat # PAB9671) and PSD-95 polyclonal antibody (Cat # PAB9623) double-label Immunostaining on 14 DIV Cortical neurons. -
Gene Info — Grin2b
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Publication Reference
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NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity.
Carroll RC, Zukin RS.
Trends in Neurosciences 2002 Nov; 25(11):571.
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Trafficking of NMDA receptors.
Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS.
Annual Review of Pharmacology and Toxicology 2002 Jan; 43:335.
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Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors.
Takasu MA, Dalva MB, Zigmond RE, Greenberg ME.
Science 2002 Jan; 295(5554):491.
Application：WB-Tr, Human, HEK 293T cells.
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NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity.
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