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- Home > Products > Polyclonal Ab > Phospho-Specific > Grin2b (phospho Y125) polyclonal antibody
Grin2b (phospho Y125) polyclonal antibody
- Catalog # : PAB9671
- Visit Frequency :
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- 100 uL
- USD $ 415
- order now, ship in 5 days
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Email : sales@abnova.com
Phone : +1-909-992-0619 (new)
Fax : +1-909-992-3401- Products will be shipped directly to the customer.
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of Grin2b.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding Y125 of rat Grin2b.
- Host:
- Rabbit
- Theoretical MW (kDa):
- 180
- Reactivity:
- Bovine, Chicken, Dog, Human, Mouse, Primates, Rat, Zebra fish
- Form:
- Liquid
- Purification:
- Affinity purification
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Publication Reference
- 1.
- NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity.
Carroll RC, Zukin RS.Trends Neurosci. 2002 Nov;25(11):571-7.
- 2.
- Trafficking of NMDA receptors.
Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS.Annu Rev Pharmacol Toxicol. 2003;43:335-58. Epub 2002 Jan 10.
- 3.
- Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors.
Takasu MA, Dalva MB, Zigmond RE, Greenberg ME.Science. 2002 Jan 18;295(5554):491-5. Epub 2001 Dec 20.
- Applications
- Western Blot (Tissue lysate)
- Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k Grin2b subunit of the NMDAR phosphorylated at Tyr1252 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Grin2b (phospho Y125) polyclonal antibody (Cat # PAB9671). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
