Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k Grin2b subunit of the NMDAR phosphorylated at Tyr1252 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Grin2b (phospho Y125) polyclonal antibody (Cat # PAB9671). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Grin2b (phospho Y125) polyclonal antibody (Cat # PAB9671) and PSD-95 polyclonal antibody (Cat # PAB9623) double-label Immunostaining on 14 DIV Cortical neurons.