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AKT2 (phospho S474) polyclonal antibody   

  • Catalog # : PAB8183
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  • 100 ug
  • USD $ 335
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT2.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S474 of human AKT2.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 60
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • Akt2 (phospho-Ser474) antibody detects endogenous levels of Akt2 only when phosphorylated at serine 474.
  • Form:
  • Liquid
  • Purification:
  • Immunoaffinity purification
  • Concentration:
  • 1 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Phospho-AKT2 Ser474 polyclonal antibody. Western blot analysis of AKT2 expression in human kidney.
  • Protocol Download
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Phospho-AKT2 Ser474 polyclonal antibody. Western blot analysis of AKT2 expression in Jurkat.
  • Protocol Download
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extract from A2780 cells using AKT2 (phospho S474) polyclonal antibody (Cat # PAB8183).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AKT2 (phospho S474) polyclonal antibody (Cat # PAB8183).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 208
  • Gene Name:
  • AKT2
  • Gene Alias:
  • PKBB,PKBBETA,PRKBB,RAC-BETA
  • Gene Description:
  • v-akt murine thymoma viral oncogene homolog 2
  • Gene Summary:
  • This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
  • Other Designations:
  • Murine thymoma viral (v-akt) homolog-2,rac protein kinase beta
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