EGFR polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of human A-431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lane 1) or treated with lambda phosphatase (lane 2) then were probed with EGFR polyclonal antibody (Cat # PAB7872).
Immunofluorescence
Immunocytochemical labeling in A-431 cells untreated (1) or treated with Calyculin A (50 nM) for 15 min (2). Cells were labeled with EGFR polyclonal antibody (Cat # PAB7872). The specificity of labeling was demonstrated with its respective blocking peptides EGFR polyclonal antibody (Cat # PAB7872) (3).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EGFR.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 961-972 of human EGFR.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This human EGFR sequence has high homology to rat and mouse EGFR, and low homology to other ErbB family members.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of human A-431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lane 1) or treated with lambda phosphatase (lane 2) then were probed with EGFR polyclonal antibody (Cat # PAB7872).Immunocytochemistry
Immunofluorescence
Immunocytochemical labeling in A-431 cells untreated (1) or treated with Calyculin A (50 nM) for 15 min (2). Cells were labeled with EGFR polyclonal antibody (Cat # PAB7872). The specificity of labeling was demonstrated with its respective blocking peptides EGFR polyclonal antibody (Cat # PAB7872) (3).Enzyme-linked Immunoabsorbent Assay
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Gene Info — EGFR
Entrez GeneID
1956Protein Accession#
P00533Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactome
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Pathway
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Disease
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Publication Reference
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Systematic analysis of the epidermal growth factor receptor by mass spectrometry reveals stimulation-dependent multisite phosphorylation.
Boeri Erba E, Bergatto E, Cabodi S, Silengo L, Tarone G, Defilippi P, Jensen ON.
Molecular & Cellular Proteomics 2005 May; 4(8):1107.
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The EGF receptor: a nexus for trafficking and signaling.
Carpenter G.
Bioessays 2000 Aug; 22(8):697.
Application：IHC, WB-Tr, Human, Rat, A-431, PC-12 cells, Mammalian cells.
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Systematic analysis of the epidermal growth factor receptor by mass spectrometry reveals stimulation-dependent multisite phosphorylation.
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