MGLL polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
MGLL polyclonal antibody (Cat # PAB7494) (0.5 ug/mL) staining of human adipose lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
293 overexpressing MGLL and probed with MGLL polyclonal antibody (Cat # PAB7494) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MGLL.
Immunogen
A synthetic peptide corresponding to human MGLL.
Sequence
C-QDLPHLVNADGQY
Host
Goat
Theoretical MW (kDa)
34.3, 33.3
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_009214.1 and NP_0010.03794.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MGLL polyclonal antibody (Cat # PAB7494) (0.5 ug/mL) staining of human adipose lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing MGLL and probed with MGLL polyclonal antibody (Cat # PAB7494) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MGLL
Entrez GeneID
11343Protein Accession#
NP_009214.1;NP_0010.03794.1Gene Name
MGLL
Gene Alias
HU-K5, HUK5, MGL
Gene Description
monoglyceride lipase
Omim ID
609699Gene Ontology
HyperlinkGene Summary
Monoglyceride lipase (MGLL; EC 3.1.1.23) functions together with hormone-sensitive lipase (LIPE; MIM 151750) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL; MIM 238600) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides (Karlsson et al., 2001 [PubMed 11470505]).[supplied by OMIM
Other Designations
lysophospholipase-like|monoacylglycerol lipase
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Interactome
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Pathway
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Disease
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Publication Reference
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A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol.
Blankman JL, Simon GM, Cravatt BF.
Chemistry & Biology 2007 Dec; 14(12):1347.
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A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol.
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