GPX3 polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of GPX3.
Immunogen
A synthetic peptide corresponding to human GPX3.
Sequence
C-NQFGKQEPGENSE
Host
Goat
Theoretical MW (kDa)
25.5
Reactivity
Human, Mouse
Specificity
This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Immunohistochemistry (3-5 ug/mL)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GPX3 polyclonal antibody (Cat # PAB7230) (1 ug/mL) staining of mouse heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GPX3 polyclonal antibody (Cat # PAB7230) (4 ug/mL) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GPX3
Entrez GeneID
2878Protein Accession#
NP_002075.2Gene Name
GPX3
Gene Alias
GPx-P, GSHPx-3, GSHPx-P
Gene Description
glutathione peroxidase 3 (plasma)
Omim ID
138321Gene Ontology
HyperlinkGene Summary
This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq
Other Designations
extracellular glutathione peroxidase|glutathione peroxidase 3
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Expression of plasma glutathione peroxidase in human liver in addition to kidney, heart, lung, and breast in humans and rodents.
Chu FF, Esworthy RS, Doroshow JH, Doan K, Liu XF.
Blood 1992 Jun; 79(12):3233.
Application:IP, Human, A498, AdrrMCF-7, HepG2, MCF-7H6 cells.
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Expression of plasma glutathione peroxidase in human liver in addition to kidney, heart, lung, and breast in humans and rodents.
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