Goat polyclonal antibody raised against synthetic peptide of ARRB2.
Immunogen:
A synthetic peptide corresponding to internal region of human ARRB2.
Sequence:
C-HDHIPLPRPQS
Host:
Goat
Theoretical MW (kDa):
46.1, 44.4
Reactivity:
Human, Mouse, Rat
Specificity:
This antibody is expected to recognize both reported isoforms NP_004304.1 and NP_945355.1. No crossreactivity is expected with Arrestin beta 1.
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Recommend Usage:
ELISA (1:32000) Western Blot (0.3-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
ARRB2 polyclonal antibody (Cat # PAB6785) (0.3 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq