APOE polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
APOE polyclonal antibody (Cat # PAB6765) (0.1 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
APOE polyclonal antibody (Cat # PAB6765) (2.5 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of APOE.
Immunogen
A synthetic peptide corresponding to human APOE.
Sequence
C-VGTSAAPVPSDNH
Host
Goat
Theoretical MW (kDa)
36.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
APOE polyclonal antibody (Cat # PAB6765) (0.1 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
APOE polyclonal antibody (Cat # PAB6765) (2.5 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — APOE
Entrez GeneID
348Protein Accession#
NP_000032.1Gene Name
APOE
Gene Alias
AD2, LPG, MGC1571, apoprotein
Gene Description
apolipoprotein E
Gene Ontology
HyperlinkGene Summary
Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq
Other Designations
apolipoprotein E3
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Interactome
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Disease
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Publication Reference
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Gene delivery of human apolipoprotein E alters brain Abeta burden in a mouse model of Alzheimer's disease.
Dodart JC, Marr RA, Koistinaho M, Gregersen BM, Malkani S, Verma IM, Paul SM.
PNAS 2005 Jan; 102(4):1211.
Application：IF, WB-Ti, Mouse, Hippocampal.
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Gene delivery of human apolipoprotein E alters brain Abeta burden in a mouse model of Alzheimer's disease.
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