APEX1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
APEX1 polyclonal antibody (Cat # PAB6125) staining (0.1 ug/mL) of A-431 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
APEX1 polyclonal antibody (Cat # PAB6125) (4 ug/mL) staining of paraffin embedded human breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of APEX1.
Immunogen
A synthetic peptide corresponding to N-terminus of human APEX1.
Sequence
PKRGKKGAVAEDGD-C
Host
Goat
Theoretical MW (kDa)
35.6
Reactivity
Human
Specificity
This antibody is expected to recognize NP_001632.2, NP_542379.1 and NP_542380.1 are varients that represent the same protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
APEX1 polyclonal antibody (Cat # PAB6125) staining (0.1 ug/mL) of A-431 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
APEX1 polyclonal antibody (Cat # PAB6125) (4 ug/mL) staining of paraffin embedded human breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — APEX1
Entrez GeneID
328Protein Accession#
NP_001632.2;NP_542379.1;NP_542380.1Gene Name
APEX1
Gene Alias
APE, APE-1, APE1, APEN, APEX, APX, HAP1, REF-1, REF1
Gene Description
APEX nuclease (multifunctional DNA repair enzyme) 1
Omim ID
107748Gene Ontology
HyperlinkGene Summary
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq
Other Designations
AP endonuclease class I|AP lyase|APEX nuclease|DNA-(apurinic or apyrimidinic site) lyase|OTTHUMP00000163988|apurinic/apyrimidinic (abasic) endonuclease|apurinic/apyrimidinic exonuclease|deoxyribonuclease (apurinic or apyrimidinic)|multifunctional DNA repa
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Interactome
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Pathway
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Disease
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Publication Reference
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Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes.
Demple B, Herman T, Chen DS.
PNAS 1991 Dec; 88(24):11450.
Application：AFC, IF, WB-Ce, WB-Re, Human, HeLa, MG63 cells, Recombinant proteins.
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Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes.
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