CDC25A polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of CDC25A polyclonal antibody (Cat # PAB4856) in mouse cerebellum tissue lysate (35 ug/lane). CDC25A (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDC25A.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S78 of human CDC25A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of CDC25A polyclonal antibody (Cat # PAB4856) in mouse cerebellum tissue lysate (35 ug/lane). CDC25A (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDC25A
Entrez GeneID
993Protein Accession#
NP_001780;P30304Gene Name
CDC25A
Gene Alias
CDC25A2
Gene Description
cell division cycle 25 homolog A (S. pombe)
Omim ID
116947Gene Ontology
HyperlinkGene Summary
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
M-phase inducer phosphatase 1|cell division cycle 25A|dual specificity phosphatase CDC25A
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Interactome
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Pathway
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Disease
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Publication Reference
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Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation.
Ito Y, Yoshida H, Matsuzuka F, Matsuura N, Nakamura Y, Nakamine H, Kakudo K, Kuma K, Miyauchi A.
International Journal of Molecular Medicine 2004 Mar; 13(3):431.
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Activation of the Raf-1/MEK/Erk kinase pathway by a novel Cdc25 inhibitor in human prostate cancer cells.
Nemoto K, Vogt A, Oguri T, Lazo JS.
The Prostate 2004 Jan; 58(1):95.
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Regulation of human Cdc25A stability by Serine 75 phosphorylation is not sufficient to activate a S phase checkpoint.
Goloudina A, Yamaguchi H, Chervyakova DB, Appella E, Fornace AJ Jr, Bulavin DV.
Cell Cycle 2003 Sep; 2(5):473.
Application：WB-Ce, WB-Tr, Human, HeLa cells.
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Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation.
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