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Last updated: 2014/11/16
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GPC3 polyclonal antibody

  • Catalog # : PAB4437
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of GPC3.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 530-560 at C-terminus of human GPC3.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of GPC3 polyclonal antibody (Cat # PAB4437) in mouse lung tissue.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Jurkat cell lysate (35 ug/lane) with GPC3 polyclonal antibody (Cat # PAB4437).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorecence staining of GPC3 polyclonal antibody (Cat # PAB4437) on HepG2 cells . The cells were acetone fixated . Antibody dilution of 1 : 50 . Original magnification 1 : 400 . Data and protocol courtesy of Dr . Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine .
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2719
  • Protein Accession#:
  • NP_004475;P51654
  • Gene Name:
  • GPC3
  • Gene Alias:
  • DGSX,OCI-5,SDYS,SGB,SGBS,SGBS1
  • Gene Description:
  • glypican 3
  • Gene Summary:
  • Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000024058,OTTHUMP00000062492,glypican proteoglycan 3
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