PHLPP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of PHLPP1 polyclonal antibody (Cat # PAB3527) in K-562 cell line lysates (35 ug/lane). PHLPP1 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PHLPP1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PHLPP1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PHLPP1 polyclonal antibody (Cat # PAB3527) in K-562 cell line lysates (35 ug/lane). PHLPP1 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution). -
Gene Info — PHLPP1
Entrez GeneID
23239Protein Accession#
O60346Gene Name
PHLPP1
Gene Alias
MGC161555, PHLPP1, PLEKHE1, SCOP, PHLPP
Gene Description
PH domain and leucine rich repeat protein phosphatase 1
Omim ID
609396Gene Ontology
HyperlinkGene Summary
family E (with leucine rich repeats) member 1; suprachiasmatic nucleus circad
Other Designations
PH domain leucine-rich repeat-containing protein phosphatase 1; PH domain-containing family E member 1; SCN circadian oscillatory protein; pleckstrin homology domain containing, family E (with leucine rich repeats) member 1; suprachiasmatic nucleus circad
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Interactome
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Disease
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Publication Reference
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The phosphatase PHLPP controls the cellular levels of protein kinase C.
Gao T, Brognard J, Newton AC.
The Journal of Biological Chemistry 2008 Mar; 283(10):6300.
Application：WB-Tr, Human, DLD-1, H157, HEK 293T, Hs578Bst, MCF-10A cells.
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PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms.
Brognard J, Sierecki E, Gao T, Newton AC.
Molecular Cell 2007 Mar; 25(6):917.
Application：IF, WB-Ce, Human, H157, HEK 293T, Hs578Bst cells.
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PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth.
Gao T, Furnari F, Newton AC.
Molecular Cell 2005 Apr; 18(1):13.
Application：WB-Tr, Human, DLD-1, H157, HT-29, HEK 293T, LN319, LN444, MDA-MB-231 cells.
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The phosphatase PHLPP controls the cellular levels of protein kinase C.
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