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- Home > Products > Polyclonal Ab > Human > Signal Transduction > ERAS polyclonal antibody
ERAS polyclonal antibody
- Catalog # : PAB3129
- Visit Frequency :
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- 100 ug
- USD $ 319
- order now, ship in 5 days
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Email : sales@abnova.com
Phone : +1-909-992-0619 (new)
Fax : +1-909-992-3401- Products will be shipped directly to the customer.
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of ERAS.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ERAS.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein A purification
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunofluorescence (1:10-50)
Immunohistochemistry (1:50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Human ERas gene has an upstream premature polyadenylation signal that results in a truncated, noncoding transcript.
Kameda T, Thomson JA.Stem Cells. 2005 Nov-Dec;23(10):1535-40. Epub 2005 Aug 4.
- 2.
- Role of ERas in promoting tumour-like properties in mouse embryonic stem cells.
Takahashi K, Mitsui K, Yamanaka S.Nature. 2003 May 29;423(6939):541-5.
- 3.
- The human c-Ha-ras2 is a processed pseudogene inactivated by numerous base substitutions.
Miyoshi J, Kagimoto M, Soeda E, Sakaki Y.Nucleic Acids Res. 1984 Feb 24;12(4):1821-8.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of ERAS polyclonal antibody (Cat # PAB3129) in K-562 cell line lysates (35 ug/lane). ERAS (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS polyclonal antibody (Cat # PAB3129) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 3266
- Protein Accession#:
- NP_853510;Q7Z444
- Gene Name:
- ERAS
- Gene Alias:
- HRAS2,HRASP,MGC126691,MGC126693
- Gene Description:
- ES cell expressed Ras
- Omim ID:
- 300437
- Gene Ontology:
- Hyperlink
- Other Designations:
- OTTHUMP00000065857,small GTPase protein E-Ras,v-Ha-ras Harvey rat sarcoma viral oncogene homolog 2,v-Ha-ras Harvey rat sarcoma viral oncogene homolog pseudogene
