CHUK polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells (lane 1) and MDA-MB-435 cells (lane 2 and 3) untreated or treated with EGF using CHUK polyclonal antibody (Cat # PAB26696).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CHUK polyclonal antibody (Cat # PAB26696).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CHUK.
Immunogen
A synthetic peptide corresponding to residues surrounding T23 of human CHUK.
Sequence
L-G-Tp-G-G
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells (lane 1) and MDA-MB-435 cells (lane 2 and 3) untreated or treated with EGF using CHUK polyclonal antibody (Cat # PAB26696).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CHUK polyclonal antibody (Cat # PAB26696). -
Gene Info — CHUK
Entrez GeneID
1147Protein Accession#
O15111Gene Name
CHUK
Gene Alias
IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16
Gene Description
conserved helix-loop-helix ubiquitous kinase
Omim ID
600664Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq
Other Designations
I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|OTTHUMP00000020273|conserved helix-loop ubiquitous kinase
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Interactome
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Pathway
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Disease
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