AKT1 (phospho S129) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S129 of human AKT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Dot Blot (1:~500)
ELISA
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C for up to 2 weeks. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot blot analysis of AKT1 (phospho S129) polyclonal antibody (Cat # PAB2612) on nitrocellulose membrane. 50 ng of phospho-peptide or non phospho-peptide per dot were adsorbed. -
Gene Info — AKT1
Entrez GeneID
207Protein Accession#
NP_005154; P31749Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Akt is a major angiogenic mediator downstream of the Ang1/Tie2 signaling pathway.
DeBusk LM, Hallahan DE, Lin PC.
Experimental Cell Research 2004 Aug; 298(1):167.
-
EGF-dependent cell cycle progression is controlled by density-dependent regulation of Akt activation.
LeVea CM, Reeder JE, Mooney RA.
Experimental Cell Research 2004 Jul; 297(1):272.
-
Phosphorylation of tau at THR212 and SER214 in human neuronal and glial cultures: the role of AKT.
Kyoung Pyo H, Lovati E, Pasinetti GM, Ksiezak-Reding H.
Neuroscience 2004 Jan; 127(3):649.
-
Akt is a major angiogenic mediator downstream of the Ang1/Tie2 signaling pathway.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com