PPT1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The PPT1 polyclonal antibody (Cat # PAB2508) is used in Western blot to detect PPT1 in 293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the PPT1 polyclonal antibody (Cat # PAB2508) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PPT1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PPT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The PPT1 polyclonal antibody (Cat # PAB2508) is used in Western blot to detect PPT1 in 293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the PPT1 polyclonal antibody (Cat # PAB2508) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — PPT1
Entrez GeneID
5538Protein Accession#
PPT1_HUMANGene Name
PPT1
Gene Alias
CLN1, INCL, PPT
Gene Description
palmitoyl-protein thioesterase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene
Other Designations
OTTHUMP00000004836|ceroid-palmitoyl-palmitoyl-protein thioesterase 1|palmitoyl-protein hydrolase 1
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Interactome
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Pathway
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Disease
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Publication Reference
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The crystal structure of palmitoyl protein thioesterase-2 (PPT2) reveals the basis for divergent substrate specificities of the two lysosomal thioesterases, PPT1 and PPT2.
Calero G, Gupta P, Nonato MC, Tandel S, Biehl ER, Hofmann SL, Clardy J.
The Journal of Biological Chemistry 2003 Sep; 278(39):37957.
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The neuronal ceroid lipofuscinoses: mutations in different proteins result in similar disease.
Weimer JM, Kriscenski-Perry E, Elshatory Y, Pearce DA.
Neuromolecular Medicine 2002 Jan; 1(2):111.
Application：IHC, WB-Tr, Human, Monkey, COS cells, Mammalian cells, Tissues.
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The crystal structure of palmitoyl protein thioesterase-2 (PPT2) reveals the basis for divergent substrate specificities of the two lysosomal thioesterases, PPT1 and PPT2.
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