MTHFR polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MTHFR.
Immunogen
A synthetic peptide corresponding to amino acids 369-383 at internal region of human MTHFR.
Sequence
C-SRPKSYIYRTQEWDE
Host
Goat
Theoretical MW (kDa)
70
Reactivity
Mouse
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:8000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
MTHFR polyclonal antibody (Cat # PAB24568) (0.3 ug/mL) staining of NIH/3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MTHFR
Entrez GeneID
4524Protein Accession#
NP_005948.3Gene Name
MTHFR
Gene Alias
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Gene Description
5,10-methylenetetrahydrofolate reductase (NADPH)
Gene Ontology
HyperlinkGene Summary
Methylenetetrahydrofolate reductase (EC 1.5.1.20) catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate for homocysteine remethylation to methionine.[supplied by OMIM
Other Designations
OTTHUMP00000002367|OTTHUMP00000002368|methylenetetrahydrofolate reductase intermediate form
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Interactome
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Pathway
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Disease
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