BID polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of BID polyclonal antibody (Cat # PAB2442) in mouse lung tissue lysates (35 ug/lane). BID (arrow) was detected using the purified polyclonal antibody.
Western Blot (Cell lysate)
The BID polyclonal antibody (Cat # PAB2442) is used in Western blot to detect BID in HL-60 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the BID polyclonal antibody (Cat # PAB2442) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with BID polyclonal antibody (Cat # PAB2442) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BID.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to the BH3 domain of human BID.
Host
Rabbit
Reactivity
Human
Specificity
BH3 Domain Specific
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of BID polyclonal antibody (Cat # PAB2442) in mouse lung tissue lysates (35 ug/lane). BID (arrow) was detected using the purified polyclonal antibody.Western Blot (Cell lysate)
The BID polyclonal antibody (Cat # PAB2442) is used in Western blot to detect BID in HL-60 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the BID polyclonal antibody (Cat # PAB2442) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with BID polyclonal antibody (Cat # PAB2442) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — BID
Entrez GeneID
637Protein Accession#
NP_001187;P55957Gene Name
BID
Gene Alias
FP497, MGC15319, MGC42355
Gene Description
BH3 interacting domain death agonist
Omim ID
601997Gene Ontology
HyperlinkGene Summary
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq
Other Designations
BH3-interacting domain death agonist|BID isoform ES(1b)|BID isoform L(2)|BID isoform Si6|Human BID coding sequence|OTTHUMP00000196197|apoptic death agonist|desmocollin type 4
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Interactome
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Pathway
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Disease
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Publication Reference
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Nonredundant role of Bax and Bak in Bid-mediated apoptosis.
Cartron PF, Juin P, Oliver L, Martin S, Meflah K, Vallette FM.
Molecular and Cellular Biology 2003 Jul; 23(13):4701.
Application：WB-Ce, Human, BeGBM, BdGBM cells.
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Caspase 8-mediated cleavage of the pro-apoptotic BCL-2 family member BID in p53-dependent apoptosis.
Fischer B, Coelho D, Dufour P, Bergerat JP, Denis JM, Gueulette J, Bischoff P.
Biochemical and Biophysical Research Communications 2003 Jun; 306(2):516.
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Necrotic cell death in response to oxidant stress involves the activation of the apoptogenic caspase-8/bid pathway.
Wang X, Ryter SW, Dai C, Tang ZL, Watkins SC, Yin XM, Song R, Choi AM.
The Journal of Biological Chemistry 2003 Aug; 278(31):29184.
Application：WB, Human, Mouse, A-549 cells, Lungs.
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Nonredundant role of Bax and Bak in Bid-mediated apoptosis.
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