ERBB2 (phospho Y1248) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) was used to detect phospho-ERBB2 in FG pancreatic carcinoma cell line lysate. FG pancreatic carcinoma cells treated with or without EGF (50 ug/mL) for 15 min ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) was used in 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Dot Blot (Peptide)
Dot blot analysis of ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) on nitrocellulose membrane. 50 ug of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of ERBB2.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y1248 of human ERBB2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:8000)
Immunohistochemistry (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) was used to detect phospho-ERBB2 in FG pancreatic carcinoma cell line lysate. FG pancreatic carcinoma cells treated with or without EGF (50 ug/mL) for 15 min ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) was used in 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Dot Blot (Peptide)
Dot blot analysis of ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) on nitrocellulose membrane. 50 ug of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. -
Gene Info — ERBB2
Entrez GeneID
2064Protein Accession#
NP_004439;P04626Gene Name
ERBB2
Gene Alias
CD340, HER-2, HER-2/neu, HER2, NEU, NGL, TKR1
Gene Description
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq
Other Designations
c-erb B2/neu protein|erbB-2|herstatin|neuroblastoma/glioblastoma derived oncogene homolog|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
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Interactome
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Pathway
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Disease
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Publication Reference
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Effect of resveratrol on the development of spontaneous mammary tumors in HER-2/neu transgenic mice.
Provinciali M, Re F, Donnini A, Orlando F, Bartozzi B, Di Stasio G, Smorlesi A.
International Journal of Cancer 2005 May; 115(1):36.
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Dietary (n-3) polyunsaturated fatty acids inhibit HER-2/neu-induced breast cancer in mice independently of the PPARgamma ligand rosiglitazone.
Yee LD, Young DC, Rosol TJ, Vanbuskirk AM, Clinton SK.
The Journal of Nutrition 2005 May; 135(5):983.
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Identification and validation of novel ERBB2 (HER2, NEU) targets including genes involved in angiogenesis.
Beckers J, Herrmann F, Rieger S, Drobyshev AL, Horsch M, Hrabé de Angelis M, Seliger B.
International Journal of Cancer 2005 Apr; 114(4):590.
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Effect of resveratrol on the development of spontaneous mammary tumors in HER-2/neu transgenic mice.
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