Goat polyclonal antibody raised against synthetic peptide of ARHGDIA.
Immunogen:
A synthetic peptide corresponding to amino acids at internal region of human ARHGDIA.
Sequence:
C-QLAQIAAENE
Host:
Goat
Theoretical MW (kDa):
28
Reactivity:
Mouse, Rat
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Recommend Usage:
ELISA (1:8000) Western Blot (1-3 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Tissue lysate)
ARHGDIA polyclonal antibody (Cat # PAB19057) (1 ug/mL) staining of mouse lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM