CDH11 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human CDH11 with C-terminal MYC tag probed with CDH11 polyclonal antibody (Cat # PAB19040) (1 ug/mL) in Lane 1 and probed with anti-MYC Tag (1/1000) in Lane 3. Mock-transfected HEK293 probed with CDH11 polyclonal antibody (Cat # PAB19040) (1 mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CDH11.
Immunogen
A synthetic peptide corresponding to amino acids 26-37 at internal region of human CDH11.
Sequence
C-ERRGHLRPSFHG
Host
Goat
Theoretical MW (kDa)
130
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:2000)
Western Blo (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human CDH11 with C-terminal MYC tag probed with CDH11 polyclonal antibody (Cat # PAB19040) (1 ug/mL) in Lane 1 and probed with anti-MYC Tag (1/1000) in Lane 3. Mock-transfected HEK293 probed with CDH11 polyclonal antibody (Cat # PAB19040) (1 mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDH11
Entrez GeneID
1009Protein Accession#
NP_001788.2Gene Name
CDH11
Gene Alias
CAD11, CDHOB, OB, OSF-4
Gene Description
cadherin 11, type 2, OB-cadherin (osteoblast)
Omim ID
600023Gene Ontology
HyperlinkGene Summary
This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. [provided by RefSeq
Other Designations
OB-cadherin|cadherin 11, type 2|cadherin-11|osteoblast cadherin
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Interactome
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Disease
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