CASP9 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, treated with Calyculin (50 nM, 30 mins), using CASP9 polyclonal antibody (Cat # PAB18314).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using CASP9 polyclonal antibody (Cat # PAB18314).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CASP9.
Immunogen
A synthetic peptide corresponding to residues surrounding S196 of human CASP9.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to CASP9.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, treated with Calyculin (50 nM, 30 mins), using CASP9 polyclonal antibody (Cat # PAB18314).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using CASP9 polyclonal antibody (Cat # PAB18314).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CASP9
Entrez GeneID
842Protein Accession#
P55211Gene Name
CASP9
Gene Alias
APAF-3, APAF3, CASPASE-9c, ICE-LAP6, MCH6
Gene Description
caspase 9, apoptosis-related cysteine peptidase
Omim ID
602234Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq
Other Designations
ICE-like apoptotic protease 6|OTTHUMP00000002322|OTTHUMP00000002323|OTTHUMP00000044594|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9|caspase 9, apoptosis-related cysteine protease
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Interactome
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Pathway
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Disease
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Publication Reference
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Mechanism of XIAP-mediated inhibition of caspase-9.
Shiozaki EN, Chai J, Rigotti DJ, Riedl SJ, Li P, Srinivasula SM, Alnemri ES, Fairman R, Shi Y.
Molecular Cell 2003 Feb; 11(2):519.
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Mechanism of XIAP-mediated inhibition of caspase-9.
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