FADD polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with PMA (125 ng/mL, 30 mins), using FADD polyclonal antibody (Cat # PAB18152).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FADD polyclonal antibody (Cat # PAB18152).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FADD.
Immunogen
A synthetic peptide corresponding to human FADD.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to FADD.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with PMA (125 ng/mL, 30 mins), using FADD polyclonal antibody (Cat # PAB18152).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FADD polyclonal antibody (Cat # PAB18152).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — FADD
Entrez GeneID
8772Protein Accession#
Q13158Gene Name
FADD
Gene Alias
GIG3, MGC8528, MORT1
Gene Description
Fas (TNFRSF6)-associated via death domain
Omim ID
602457Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq
Other Designations
Fas-associated via death domain|Fas-associating death domain-containing protein|Fas-associating protein with death domain|growth-inhibiting gene 3 protein|mediator of receptor-induced toxicity
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Interactome
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Pathway
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Disease
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Publication Reference
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Phosphorylated FADD induces NF-kappaB, perturbs cell cycle, and is associated with poor outcome in lung adenocarcinomas.
Chen G, Bhojani MS, Heaford AC, Chang DC, Laxman B, Thomas DG, Griffin LB, Yu J, Coppola JM, Giordano TJ, Lin L, Adams D, Orringer MB, Ross BD, Beer DG, Rehemtulla A.
PNAS 2005 Aug; 102(35):12507.
Application：IHC-P, WB-Ti, Human, Human lung cancer, Jurkat cells.
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FADD is required for DR4- and DR5-mediated apoptosis: lack of trail-induced apoptosis in FADD-deficient mouse embryonic fibroblasts.
Kuang AA, Diehl GE, Zhang J, Winoto A.
The Journal of Biological Chemistry 2000 Aug; 275(33):25065.
Application：WB-Ce, Mouse, MEFs.
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Phosphorylation of FADD/ MORT1 at serine 194 and association with a 70-kDa cell cycle-regulated protein kinase.
Scaffidi C, Volkland J, Blomberg I, Hoffmann I, Krammer PH, Peter ME.
The Journal of Immunology 2000 Feb; 164(3):1236.
Application：IP, IP-WB, WB-Tr, Human, BJAB, 293T cells.
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Phosphorylated FADD induces NF-kappaB, perturbs cell cycle, and is associated with poor outcome in lung adenocarcinomas.
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