PTK2B polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PTK2B.
Immunogen
A synthetic peptide corresponding to human PTK2B.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to PTK2B.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells treated with UV (15 mins), using PTK2B polyclonal antibody (Cat # PAB18133).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using PTK2B polyclonal antibody (Cat # PAB18133).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
-
Gene Info — PTK2B
Entrez GeneID
2185Protein Accession#
Q14289Gene Name
PTK2B
Gene Alias
CADTK, CAKB, FADK2, FAK2, FRNK, PKB, PTK, PYK2, RAFTK
Gene Description
PTK2B protein tyrosine kinase 2 beta
Omim ID
601212Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CAK beta|OTTHUMP00000128275|OTTHUMP00000162913|calcium-dependent tyrosine kinase|cell adhesion kinase beta|focal adhesion kinase 2|proline-rich tyrosine kinase 2|protein kinase B|protein tyrosine kinase 2 beta|related adhesion focal tyrosine kinase
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Roles of protein kinase C, Ca2+, Pyk2, and c-Src in agonist activation of rat lacrimal gland p42/p44 MAPK.
Hodges RR, Rios JD, Vrouvlianis J, Ota I, Zoukhri D, Dartt DA.
Investigative Ophthalmology & Visual Science 2006 Aug; 47(8):3352.
Application:WB, Rat, Rat Lacrimal Gland Acini.
-
The Rap GTPases regulate integrin-mediated adhesion, cell spreading, actin polymerization, and Pyk2 tyrosine phosphorylation in B lymphocytes.
McLeod SJ, Shum AJ, Lee RL, Takei F, Gold MR.
The Journal of Biological Chemistry 2004 Mar; 279(13):12009.
Application:IP, WB, Mouse, A20 cells.
-
G13alpha-mediated PYK2 activation. PYK2 is a mediator of G13alpha -induced serum response element-dependent transcription.
Shi CS, Sinnarajah S, Cho H, Kozasa T, Kehrl JH.
The Journal of Biological Chemistry 2000 Aug; 275(32):24470.
Application:WB-Tr, Human, HeLa cells.
-
Roles of protein kinase C, Ca2+, Pyk2, and c-Src in agonist activation of rat lacrimal gland p42/p44 MAPK.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com