Rabbit polyclonal antibody raised against synthetic peptide of ADD1.
Immunogen:
A synthetic peptide corresponding to residues surrounding S726 of human ADD1.
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Specificity:
This antibody is specific to ADD1.
Form:
Liquid
Recommend Usage:
Western Blot (1:500~1:1000) Immunohistochemistry (1:50~1:100) Immunofluorescence (1:500~1:1000) ELISA (1:40000) The optimal working dilution should be determined by the end user.
Western blot analysis of extracts from HeLa cells treated with Forskolin (40 nM, 30 min), using ADD1 polyclonal antibody (Cat # PAB18057). Peptide "+" means "with peptide blocking".
Immunohistochemical analysis of paraffin-embedded human brain tissue using ADD1 polyclonal antibody (Cat # PAB18057). Peptide "+" means "with peptide blocking".
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq