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Last updated: 2014/7/21
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IL6 polyclonal antibody

  • Catalog # : PAB16919
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant IL6.
  • Immunogen:
  • Recombinant protein corresponding to full length human IL6.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Lyophilized
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:300-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot using IL6 polyclonal antibody (Cat # PAB16919) shows detection of a band ~24 KDa in size corresponding to recombinant human IL6 (arrowhead). Molecular weight markers are also shown. After transfer, the membrane was blocked overnight with 1% BSA in TTBS followed by reaction with primary antibody at a 1 : 500 dilution. Detection occurred using HRP conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 in blocking buffer and FemtoMax™ Chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3569
  • Gene Name:
  • IL6
  • Gene Alias:
  • BSF2,HGF,HSF,IFNB2,IL-6
  • Gene Description:
  • interleukin 6 (interferon, beta 2)
  • Gene Summary:
  • This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq
  • Other Designations:
  • interleukin 6
  • Related Disease
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