Ccl2 polyclonal antibody
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More Files
- More Functions
Immunohistochemistry
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of obese mouse proximal tubule tissue.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant mouse Ccl2.
Immunogen
Recombinant protein corresponding to full length mouse Ccl2.
Host
Rabbit
Reactivity
Mouse, Rat
Form
Lyophilized
Purification
Protein G affinity chromatography
Recommend Usage
ELISA (0.5-2 ug/mL), detection limit 2-4 ng/mL using recombinant mouse CCL2
Immunohistochemistry (2-10 ug/mL)
Western Blot (0.1-0.2 ug/mL), detection limit 1.5-3 ng/lane using recombinant mouse CCL2
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH7.4.
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 0.01 M PBS pH 7.4 or other diluents, store at 4°C for 1 month or store at -20°C for 12 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of obese mouse proximal tubule tissue.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ccl2
Entrez GeneID
20296Protein Accession#
P10148Gene Name
Ccl2
Gene Alias
AI323594, HC11, JE, MCAF, MCP-1, MCP1, SMC-CF, Scya2, Sigje
Gene Description
chemokine (C-C motif) ligand 2
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000000786|monocyte chemoattractant protein-1|monocyte chemotactic protein|small inducible cytokine A2
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Publication Reference
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Herbal Medication, Macmoondong Decoction, Attenuates LPS-Induced COPD in Small Airways via TGF-β, CCL-2, and CXCL1.
Soon-Young Lee, Bossng Kang, Chun-Sik Bae, Seung-Sik Cho, Dae-Hun Park.
Evidence-Based Complementary and Alternative Medicine : ECAM. 2020 Apr; 2020:6413.
Application：IHC-P, Mouse, Lung.
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Immunomodulation by adoptive regulatory T-cell transfer improves Coxsackievirus B3-induced myocarditis.
Pappritz K, Savvatis K, Miteva K, Kerim B, Dong F, Fechner H, Müller I, Brandt C, Lopez B, González A, Ravassa S, Klingel K, Diez J, Reinke P, Volk HD, Van Linthout S, Tschöpe C.
FASEB Journal 2018 Jun; [Epub].
Application：IHC-Fr, Mouse, Heart.
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Establishment of a chronic obstructive pulmonary disease mouse model based on the elapsed time after LPS intranasal instillation.
Lee SY, Cho JH, Cho SS, Bae CS, Kim GY, Park DH.
Laboratory Animal Research 2018 Mar; 34(1):1.
Application：IHC-P, Mouse, Lung.
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Association of the MCP-1 -2518 A/G polymorphism and no association of its receptor CCR2 -64 V/I polymorphism with lupus nephritis.
Malafronte P, Vieira JM Jr, Pereira AC, Krieger JE, Barros RT, Woronik V.
The Journal of Rheumatology 2010 Mar; 37(4):776.
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Rationally evolving MCP-1/CCL2 into a decoy protein with potent anti-inflammatory activity in vivo.
Piccinini AM, Knebl K, Rek A, Wildner G, Diedrichs-Mohring M, Kungl AJ.
The Journal of Biological Chemistry 2010 Jan; 285(12):8782.
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MCP-1/CCL2 modifies axon properties in a PMP22-overexpressing mouse model for Charcot-Marie-tooth 1A neuropathy.
Kohl B, Fischer S, Groh J, Wessig C, Martini R.
The American Journal of Pathology 2010 Jan; 176(3):1390.
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Herbal Medication, Macmoondong Decoction, Attenuates LPS-Induced COPD in Small Airways via TGF-β, CCL-2, and CXCL1.
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