CASP9 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant CASP9.
Immunogen
Recombinant protein corresponding to human CASP9.
Host
Rabbit
Reactivity
Dog, Gerbils, Human, Mouse, Rat
Specificity
Caspase-9 immunostaining may appear diffuse or punctate. It may be localized in the cytosol, mitochondria, and nucleus. Caspase-9 staining in the nucleus is considered to be an indication of active Caspase-9. Both pro and active Caspase-9 staining may also be seen in the mitochondria. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation.
Form
Liquid
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:5000)
Immunoprecipitation (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed, paraffin-embedded human brain sections stained for Active/Cleaved CASP9 expression using CASP9 polyclonal antibody (Cat #PAB14669) at 1 : 2000. A and B. The pattern of CASP9 staining may vary between different types of neurons. Hematoxylin-eosin counterstain.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed, paraffin-embedded dog brain sections stained for Active/Cleaved CASP9 expression using CASP9 polyclonal antibody (Cat #PAB14669) at 1 : 2000. A and B. The pattern of CASP9 staining may vary between different types of neurons. Hematoxylin-eosin counterstain.Immunohistochemistry (Frozen sections)
Immunoprecipitation
Immunoelectron Microscopy
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Gene Info — CASP9
Entrez GeneID
842Protein Accession#
NP_001220Gene Name
CASP9
Gene Alias
APAF-3, APAF3, CASPASE-9c, ICE-LAP6, MCH6
Gene Description
caspase 9, apoptosis-related cysteine peptidase
Omim ID
602234Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq
Other Designations
ICE-like apoptotic protease 6|OTTHUMP00000002322|OTTHUMP00000002323|OTTHUMP00000044594|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9|caspase 9, apoptosis-related cysteine protease
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Interactome
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Pathway
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Disease
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Publication Reference
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Caspases: pharmacological manipulation of cell death.
Lavrik IN, Golks A, Krammer PH.
The Journal of Clinical Investigation 2005 Oct; 115(10):2665.
Application:WB-Tr, Human, Human mammalian cells.
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Identifying the practice characteristics of advanced practitioners in acute and critical care settings.
Woods LP.
Intensive & Critical Care Nursing 1998 Dec; 14(6):308.
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Caspases: pharmacological manipulation of cell death.
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