Rabbit polyclonal antibody raised against synthetic peptide of Dnmt3a.
A mixture of synthetic peptides (conjugated with KLH) corresponding to internal region of mouse Dnmt3a.
ELISA (1:1000-1:10000) Western Blot (1:500) The optimal working dilution should be determined by the end user.
In serum (0.05% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Cell lysate)
Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using Dnmt3a polyclonal antibody (Cat # PAB14074) diluted 1 : 500 in TBS-Tween containing 5% skimmed milk.
ELISA was performed using a serial dilution of Dnmt3a polyclonal antibody (Cat # PAB14074). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 86,500.