Rabbit polyclonal antibody raised against recombinant PCNA.
Immunogen:
Recombinant protein corresponding to human PCNA.
Host:
Rabbit
Reactivity:
Human
Specificity:
This antibody recognizes human PCNA.
Form:
Liquid
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
Recommend Usage:
Western Blot (0.1-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-5 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2 (10% Proclin300)
Storage Instruction:
Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing.
Immunohistochemical staining of human tonsil tissue stained with PCNA polyclonal antibody (Cat # PAB12722) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq
Other Designations:
DNA polymerase delta auxiliary protein,OTTHUMP00000030189,OTTHUMP00000030190,cyclin