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NF1 polyclonal antibody   

  • Catalog # : PAB12636
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  • 100 ug
  • USD $ 319
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of NF1.
  • Immunogen:
  • A synthetic peptide corresponding to residues surrounding S2741 of human NF1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 240
  • Reactivity:
  • Bovine, Dog, Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes ~240 KDa of human NF1 without the phosphorylation site of Ser 2741. It does not cross react to phospho NF1.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • The tissue lysates derived from hypoxic mouse brain were immunoblotted by NF1 polyclonal antibody (Cat # PAB12636) at 1 : 1000.
  • Immunoprecipitation
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot Blot : 1 ug peptide was blot onto NC membrane.
    A : NF1 (pS2741).
    B : NF1 (Non-phospho).
    C : Non-related phospho-specific phophopeptide.
    Followed were blotted at a 1 : 1000 dilution by :
    1. Phospho-NF1 S2741 polyclonal antibody (Cat # PAB12637).
    2. NF1 polyclonal antibody (Cat # PAB12636).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4763
  • Gene Name:
  • NF1
  • Gene Alias:
  • DKFZp686J1293,FLJ21220,NFNS,VRNF,WSS
  • Gene Description:
  • neurofibromin 1
  • Gene Summary:
  • This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq
  • Other Designations:
  • neurofibromin
  • Gene Pathway
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