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Western Blot (Cell lysate)

Western blot using CCNT2 polyclonal antibody (Cat # PAB11292) is shown to detect two major bands (arrowheads) corresponding to human CCN T2a and T2b as indicated.
Approximately 33 ug of a HeLa whole cell lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking the membrane with 5% BLOTTO in PBS, the membrane was probed forovernight at 4°C with the primary antibody diluted to 1 : 500 in 5% BLOTTO in PBS.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red).
IRDye™ 800 fluorescenceimage was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is atrademark of LI-COR, Inc.

Immunoprecipitation

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

905

Protein Accession#

NP_001232(isoforma);NP_490595(isoformb)

Gene Name

CCNT2

Gene Alias

FLJ90560, MGC134840

Gene Description

cyclin T2

Omim ID

603862

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. Two alternatively spliced transcript variants, which encode distinct isoforms, have been described. [provided by RefSeq

Other Designations

SDS-stable vimentin-bound DNA fragment HEF42VIM22|cyclin T2a|cyclin T2b|subunit of positive elongation transcription factor b