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Last updated: 2014/10/27
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CCNT2 polyclonal antibody

  • Catalog # : PAB11292
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant CCNT2.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-584 of human CCNT2.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Dog, Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In antiserum (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using CCNT2 polyclonal antibody (Cat # PAB11292) is shown to detect two major bands (arrowheads) corresponding to human CCN T2a and T2b as indicated.
    Approximately 33 ug of a HeLa whole cell lysate was separated by 4-20% Tris Glycine SDS-PAGE.
    After blocking the membrane with 5% BLOTTO in PBS, the membrane was probed forovernight at 4°C with the primary antibody diluted to 1 : 500 in 5% BLOTTO in PBS.
    The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red).
    IRDye™ 800 fluorescenceimage was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is atrademark of LI-COR, Inc.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 905
  • Protein Accession#:
  • NP_001232(isoforma);NP_490595(isoformb)
  • Gene Name:
  • CCNT2
  • Gene Alias:
  • FLJ90560,MGC134840
  • Gene Description:
  • cyclin T2
  • Gene Summary:
  • The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. Two alternatively spliced transcript variants, which encode distinct isoforms, have been described. [provided by RefSeq
  • Other Designations:
  • SDS-stable vimentin-bound DNA fragment HEF42VIM22,cyclin T2a,cyclin T2b,subunit of positive elongation transcription factor b
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