BDNF polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot (Tissue lysate) analysis of BDNF expression in human brain.
Monomeric BDNF at 14 kDa in all samples, as well as monomeric proBDNF at 32 kDa in human brain.
Western Blot (Cell lysate)
Western blot (Cell lysate) analysis of BDNF expression of SH-SY5Y cell lysates.
Monomeric BDNF at 14 kDa in all samples, as well as monomeric proBDNF at 32 kDa in cell lysates prepared in RIPA buffer.
Western Blot
Western blot analysis of BDNF expression in human serum.
Monomeric BDNF at 14 kDa in all samples, and BDNF isoform at 18 kDa in human serum.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BDNF.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 129-138 human BDNF.
Sequence
HSDPARRGEL
Host
Rabbit
Reactivity
Human, Rat
Specificity
Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis.
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:1000-1:2000)
ELISA (1:1000-1:2000)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from whole serum.
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 100 uL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot (Tissue lysate) analysis of BDNF expression in human brain.
Monomeric BDNF at 14 kDa in all samples, as well as monomeric proBDNF at 32 kDa in human brain.Western Blot (Cell lysate)
Western blot (Cell lysate) analysis of BDNF expression of SH-SY5Y cell lysates.
Monomeric BDNF at 14 kDa in all samples, as well as monomeric proBDNF at 32 kDa in cell lysates prepared in RIPA buffer.Western Blot
Western blot analysis of BDNF expression in human serum.
Monomeric BDNF at 14 kDa in all samples, and BDNF isoform at 18 kDa in human serum.Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
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Gene Info — BDNF
Entrez GeneID
627Protein Accession#
P23560Gene Name
BDNF
Gene Alias
MGC34632
Gene Description
brain-derived neurotrophic factor
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq
Other Designations
neurotrophin
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Interactome
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Pathway
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Disease
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