CDC25A (phospho S75) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The CDC25A (phospho S75) polyclonal antibody (Cat # PAB0429) is used in Western blot to detect Phospho-CDC25A-S75 in mouse kidney tissue lysate.
Western Blot (Cell lysate)
The CDC25A (phospho S75) polyclonal antibody (Cat # PAB0429) is used in Western blot to detect Phospho-CDC25A-S75 in Hela (left) and HL-60 (right) cell lysates.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC25A (phospho S75) polyclonal antibody (Cat # PAB0429) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC25A.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S75 of human CDC25A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The CDC25A (phospho S75) polyclonal antibody (Cat # PAB0429) is used in Western blot to detect Phospho-CDC25A-S75 in mouse kidney tissue lysate.Western Blot (Cell lysate)
The CDC25A (phospho S75) polyclonal antibody (Cat # PAB0429) is used in Western blot to detect Phospho-CDC25A-S75 in Hela (left) and HL-60 (right) cell lysates.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC25A (phospho S75) polyclonal antibody (Cat # PAB0429) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — CDC25A
Entrez GeneID
993Protein Accession#
NP_001780;P30304Gene Name
CDC25A
Gene Alias
CDC25A2
Gene Description
cell division cycle 25 homolog A (S. pombe)
Omim ID
116947Gene Ontology
HyperlinkGene Summary
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
M-phase inducer phosphatase 1|cell division cycle 25A|dual specificity phosphatase CDC25A
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Interactome
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Pathway
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Disease
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Publication Reference
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Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation.
Ito Y, Yoshida H, Matsuzuka F, Matsuura N, Nakamura Y, Nakamine H, Kakudo K, Kuma K, Miyauchi A.
International Journal of Molecular Medicine 2004 Mar; 13(3):431.
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Activation of the Raf-1/MEK/Erk kinase pathway by a novel Cdc25 inhibitor in human prostate cancer cells.
Nemoto K, Vogt A, Oguri T, Lazo JS.
The Prostate 2004 Jan; 58(1):95.
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Regulation of human Cdc25A stability by Serine 75 phosphorylation is not sufficient to activate a S phase checkpoint.
Goloudina A, Yamaguchi H, Chervyakova DB, Appella E, Fornace AJ Jr, Bulavin DV.
Cell Cycle 2003 Sep; 2(5):473.
Application：WB-Ce, WB-Tr, Human, HeLa cells.
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Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation.
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