CASP9 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of CASP9. Various tissue lysates were prepared from human autopsy material and normalized for total protein. Most tissues contained the ~50 KDa pro-CASP9 protein. Cleaved CASP9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9 : recombinant human pro-CASP9 (full-length) protein. PRO : pro-CASP9. L : large subunit of cleaved CASP9. S : small subunit of cleaved CASP9.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of CASP9 expression in formalin-fixed, paraffin-embedded mouse brain tissue sections. Using CASP9 polyclonal antibody (Cat # PAB0248) at 1 : 2000. Hematoxylin-eosin counterstain. A : Brain striatum. B : Brain stem motor neuron.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant CASP9.
Immunogen
Recombinant protein corresponding to full length human CASP9.
Host
Rabbit
Reactivity
Dog, Gerbils, Human, Mouse, Rat
Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of CASP9. Various tissue lysates were prepared from human autopsy material and normalized for total protein. Most tissues contained the ~50 KDa pro-CASP9 protein. Cleaved CASP9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9 : recombinant human pro-CASP9 (full-length) protein. PRO : pro-CASP9. L : large subunit of cleaved CASP9. S : small subunit of cleaved CASP9.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of CASP9 expression in formalin-fixed, paraffin-embedded mouse brain tissue sections. Using CASP9 polyclonal antibody (Cat # PAB0248) at 1 : 2000. Hematoxylin-eosin counterstain. A : Brain striatum. B : Brain stem motor neuron.Immunohistochemistry (Frozen sections)
Immunoprecipitation
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Gene Info — CASP9
Entrez GeneID
842Gene Name
CASP9
Gene Alias
APAF-3, APAF3, CASPASE-9c, ICE-LAP6, MCH6
Gene Description
caspase 9, apoptosis-related cysteine peptidase
Omim ID
602234Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq
Other Designations
ICE-like apoptotic protease 6|OTTHUMP00000002322|OTTHUMP00000002323|OTTHUMP00000044594|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9|caspase 9, apoptosis-related cysteine protease
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Interactome
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Pathway
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Disease
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Publication Reference
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Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.
Krajewska M, Rosenthal RE, Mikolajczyk J, Stennicke HR, Wiesenthal T, Mai J, Naito M, Salvesen GS, Reed JC, Fiskum G, Krajewski S.
Experimental Neurology 2004 Oct; 189(2):261.
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Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.
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