LIF (Human) Recombinant Protein
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More Files
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Western Blot (Recombinant protein)
Western blot analysis of LIF (Human) Recombinant Protein (Cat # P5882) using protein specific antibody.
Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human LIF (196 a.a.) full-lenth recombinant protein with His tag expressed in Barley grain (Hordeum vulgare). This protein is endotoxins-free and thus optimal to use in stem cell cultures.
Host
Plants
Theoretical MW (kDa)
25-30
Reactivity
Human
Form
Lyophilized
Preparation Method
Hordeum vulgare (barley) expression system
Purification
Chromatography
Purity
> 95% by SDS-PAGE
Endotoxin Level
Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by turbidimetric kineticassay.
Activity
Activity has been measured by cell-based proliferation assay using TF-1 cells. ED50 is 0.36 - 0.55 ng/mL
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from PBS, pH 7.2
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot (Recombinant protein)
Western blot analysis of LIF (Human) Recombinant Protein (Cat # P5882) using protein specific antibody.Functional Study
SDS-PAGE
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Gene Info — LIF
Entrez GeneID
3976Gene Name
LIF
Gene Alias
CDF, DIA, HILDA
Gene Description
leukemia inhibitory factor (cholinergic differentiation factor)
Omim ID
159540Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. [provided by RefSeq
Other Designations
D factor|cholinergic differentiation factor|differentiation inhibitory activity|differentiation stimulating factor
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Interactome
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