GDNF (Human) Recombinant Protein
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More Files
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Western Blot (Recombinant protein)
Western blot analysis of GDNF (Human) Recombinant Protein (Cat # P5881) using protein specific antibody.
Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human GDNF (150 a.a.) full-lenth recombinant protein with His tag expressed in Barley grain (Hordeum vulgare). This protein is endotoxins-free and thus optimal to use in stem cell cultures.
Host
Plants
Theoretical MW (kDa)
25
Reactivity
Human
Form
Lyophilized
Preparation Method
Hordeum vulgare (barley) expression system
Purification
Chromatography
Purity
> 95% by SDS-PAGE
Endotoxin Level
Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by turbidimetric kineticassay.
Activity
Activity has been measured by cell-based proliferation assay using rat C6 cells. ED50 is 1.96 ug/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from PBS, pH 7.2
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot (Recombinant protein)
Western blot analysis of GDNF (Human) Recombinant Protein (Cat # P5881) using protein specific antibody.Functional Study
SDS-PAGE
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Gene Info — GDNF
Entrez GeneID
2668Gene Name
GDNF
Gene Alias
ATF1, ATF2, HFB1-GDNF
Gene Description
glial cell derived neurotrophic factor
Gene Ontology
HyperlinkGene Summary
This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq
Other Designations
astrocyte-derived trophic factor|glial cell line derived neurotrophic factor|glial derived neurotrophic factor
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Interactome
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Disease
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