FGF2 (Human) Recombinant Protein
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Specification
Product Description
Human FGF2 (146 a.a.) partial recombinant protein with ERHV-His tag expressed in Barley grain (Hordeum vulgare).
Host
Plants
Theoretical MW (kDa)
17.8
Form
Lyophilized
Preparation Method
Barley grain (Hordeum vulgare) expression system
Purification
Chromatography
Concentration
100 ug/mL
Purity
> 95% by SDS-PAGE
Endotoxin Level
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
Activity
The bioactivity of recombinant human FGFB is determined by measuring the dose dependent effect on the proliferation of 3T3 cells. The ED50 for this effect using FGF basic 146 is typically < 0.4 ng/mL, which corresponds to a specific activity > 2.5 x106 U/mg.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from PBS pH 7.2
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot (Recombinant protein)
Western blot analysis of FGF2 (Human) Recombinant Protein (Cat # P5824) using protein specific antibody.Functional Study
SDS-PAGE
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Gene Info — FGF2
Entrez GeneID
2247Protein Accession#
NM_002006Gene Name
FGF2
Gene Alias
BFGF, FGFB, HBGF-2
Gene Description
fibroblast growth factor 2 (basic)
Omim ID
134920Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq
Other Designations
basic fibroblast growth factor bFGF|fibroblast growth factor 2|heparin-binding growth factor 2|prostatropin
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