IL4 (Human) Recombinant Protein
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More Files
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Western Blot (Recombinant protein)
Western blot analysis of IL4 (Human) Recombinant Protein (Cat # P5823) using protein specific antibody.
Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human IL4 (130 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
Host
Plants
Theoretical MW (kDa)
17.2
Form
Lyophilized
Preparation Method
Barley grain (Hordeum vulgare) expression system
Purification
Chromatography
Concentration
100 ug/mL
Purity
> 95% by SDS-PAGE
Endotoxin Level
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
Activity
The bioactivity is determined by the cell proliferation assay for IL4 for dose-dependent cell proliferation of human TF-1 cells. The ED50 value for this effect is typically < 0.3 ng/mL, corresponding to a specific activity > 3.3 x 10e6 units/mg. Optimal concentration should be determined for specific applications and cell lines.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from PBS, pH7,2.
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot (Recombinant protein)
Western blot analysis of IL4 (Human) Recombinant Protein (Cat # P5823) using protein specific antibody.Functional Study
SDS-PAGE
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Gene Info — IL4
Entrez GeneID
3565Protein Accession#
NP_000580Gene Name
IL4
Gene Alias
BCGF-1, BCGF1, BSF1, IL-4, MGC79402
Gene Description
interleukin 4
Omim ID
147780Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
B cell growth factor 1|B_cell stimulatory factor 1|lymphocyte stimulatory factor 1
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