VEGFA (Human) Recombinant Protein
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Specification
Product Description
Human VEGFA (165 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
Host
Plants
Theoretical MW (kDa)
30
Form
Lyophilized
Preparation Method
Barley grain (Hordeum vulgare) expression system
Purification
Chromatography
Concentration
100 ug/mL
Purity
> 95% by SDS-PAGE
Endotoxin Level
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
Activity
The ED50 is determined by Cell Proliferation Assay for the dose-dependent effects of serial dilutions of recombinant human VEGFA on HUVEC cells. The ED50 value was determined to be 0.58 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from PBS, pH 7.2
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot (Recombinant protein)
Western blot analysis of VEGFA (Human) Recombinant Protein (Cat # P5816) using protein specific antibody.Functional Study
SDS-PAGE
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Gene Info — VEGFA
Entrez GeneID
7422Protein Accession#
NP_001165097Gene Name
VEGFA
Gene Alias
MGC70609, VEGF, VEGF-A, VPF
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
Other Designations
vascular endothelial growth factor isoform VEGF165|vascular permeability factor
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