FLT3LG (Human) Recombinant Protein
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More Files
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Western Blot (Recombinant protein)
Western blot analysis of FLT3LG (Human) Recombinant Protein (Cat # P5815) using protein specific antibody.
Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human FLT3LG (156 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
Host
Plants
Theoretical MW (kDa)
20, 22
Form
Lyophilized
Preparation Method
Barley grain (Hordeum vulgare) expression system
Purification
Chromatography
Concentration
100 ug/mL
Purity
> 98% by SDS-PAGE
Endotoxin Level
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
Activity
The ED50 in the range of 0.12-0.18 ng/mL, corresponding to specific activity 6.62 X 106 U/mg. We recommend that the optimal concentratio for each specific aplication be determined by an initial dose-response assay.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from 10 mM acetic acid, pH 5.0
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot (Recombinant protein)
Western blot analysis of FLT3LG (Human) Recombinant Protein (Cat # P5815) using protein specific antibody.Functional Study
SDS-PAGE
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Gene Info — FLT3LG
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Interactome
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Pathway
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Disease
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