Lif (Mouse) Recombinant Protein
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More Files
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Western Blot (Recombinant protein)
Western blot analysis of Lif (Mouse) Recombinant Protein (Cat # P5813) using protein specific antibody.
Result of activity analysis
Result of activity analysis
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Specification
Product Description
Mouse Lif (188 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
Host
Plants
Theoretical MW (kDa)
28-30
Form
Lyophilized
Preparation Method
Barley grain (Hordeum vulgare) expression system
Purification
Chromatography
Concentration
100 ug/mL
Purity
> 98% by SDS-PAGE
Endotoxin Level
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
Activity
The bioactivity was determined by the ability to induce differentiation of murine M1 myeloid leukemia cells. The ED50 value for this effect is typically < 1 ng/mL, corresponding to a specific activity of > 5 x 107 U/mg (where 50 U is defined as the amount of mouse Lif required to induce differentiation of 50% of M1 cells).
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from PBS, pH 7.2
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot (Recombinant protein)
Western blot analysis of Lif (Mouse) Recombinant Protein (Cat # P5813) using protein specific antibody.Functional Study
SDS-PAGE
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Gene Info — Lif
Entrez GeneID
16878Protein Accession#
AAA37211.1Gene Name
Lif
Gene Alias
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Gene Description
leukemia inhibitory factor
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000005253
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