CDKN2A monoclonal antibody, clone 5A8A4,3G8D12
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Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant CDKN2A.
Immunogen
Recombinant protein corresponding to truncated human CDKN2A.
Host
Mouse
Reactivity
Human
Form
Liquid
Recommend Usage
Western Blot (1:500-1:2000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot analysis using CDKN2A monoclonal antibody, clone 5A8A4, 3G8D12 (Cat # MAB8309) against truncated CDKN2A recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human normal, hyperplastic and tumor tissue showing nuclear location using CDKN2A monoclonal antibody, clone 5A8A4, 3G8D12 (Cat # MAB8309) with DAB staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDKN2A
Entrez GeneID
1029Gene Name
CDKN2A
Gene Alias
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, TP16, p14, p14ARF, p16, p16INK4, p16INK4a, p19
Gene Description
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
Gene Ontology
HyperlinkGene Summary
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq
Other Designations
CDK4 inhibitor p16-INK4|OTTHUMP00000021147|OTTHUMP00000021148|cell cycle negative regulator beta|cyclin-dependent kinase inhibitor 2A|cyclin-dependent kinase inhibitor p16|multiple tumor suppressor 1
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Comparative Transcriptomic Profiling in HPV-associated Cervical Carcinogenesis: Implication of MHC Class II and Immunoglobulin Heavy Chain Genes.
Shandra Devi Balasubramaniam, Kah Keng Wong, Chern Ein Oon, Venugopal Balakrishnan, Gurjeet Kaur.
Life Sciences 2020 Sep; 256:118026.
Application:IHC-P, Human, Human cervix, Human squamous cell carcinoma.
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Haploinsufficiency of p18(INK4c) sensitizes mice to carcinogen-induced tumorigenesis.
Bai F, Pei XH, Godfrey VL, Xiong Y.
Molecular and Cellular Biology 2003 Feb; 23(4):1269.
Application:IHC-P, Mouse, Abdominal cancer, Liver cancer, Lung cancer .
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Tumor suppression by Ink4a-Arf: progress and puzzles.
Lowe SW, Sherr CJ.
Biochemistry 2003 Feb; 13(1):77.
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The INK4a/ARF network in tumour suppression.
Sherr CJ.
Nat Rev Mol Cell Biol 2001 Oct; 2(10):731.
Application:IHC, WB-Tr, Human, Mouse, Cancers, Mammalian cells.
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Comparative Transcriptomic Profiling in HPV-associated Cervical Carcinogenesis: Implication of MHC Class II and Immunoglobulin Heavy Chain Genes.
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